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Science 22 February 1980:
Vol. 207. no. 4433, pp. 889 - 891
DOI: 10.1126/science.7355268
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Articles

Science, Vol 207, Issue 4433, 889-891
Copyright © 1980 by American Association for the Advancement of Science

articles

Cellular senescence in a cloned strain of bovine fetal aortic endothelial cells

SN Mueller, EM Rosen, and EM Levine

The life-span in vitro and other proliferative characteristics of a strain of endothelial cells cloned from the aorta of a fetal calf were examined. Cultures of these cells had a replicative life-span of approximately 80 cumulative population doublings. Growth rates in the logarithmic phase and plateau densities decreased as the cumulative population-doubling level increased. After approximately 65 percent of the life-span of a culture was completed, the percentage of cells that incorporated [3H]thymidine during a 24-hour labeling period began to decrease rapidly. The cells expressed factor VIII antigen and their intercellular borders were stainable with silver nitrate throughout the life-span of each culture. Average cellular attachment size increased more than threefold between cumulative population-doubling levels 41 and 80. The facility with which cloned strains of endothelial cells can be isolated should encourage further exploitation of this important cell culture model.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Human endothelial cells: use of heparin in cloning and long-term serial cultivation.
S. Thornton, S. Mueller, and E. Levine (1983)
Science 222, 623-625
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