Science, Vol 207, Issue 4427, 199-201
Copyright © 1980 by American Association for the Advancement of Science
Time: a new parameter for kinetic measurements in flow cytometry
JC Martin
and
DE Swartzendruber
The measure of time was used as an additional parameter on an existing flow cytometer to study the kinetics of enzyme activities and cell-stain interactions. By correlating all fluorescent signals from single cells with time, the dynamics of a reaction can be followed for several minutes. This advanced application of flow cytometry is easily implemented and can be incorporated into any flow cytometer that has two-parameter analysis capability.