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Science 11 January 1980:
Vol. 207. no. 4427, pp. 189 - 191
DOI: 10.1126/science.6243190
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Articles

Science, Vol 207, Issue 4427, 189-191
Copyright © 1980 by American Association for the Advancement of Science

articles

Human rotavirus type 2: cultivation in vitro

RG Wyatt, WD James, EH Bohl, KW Theil, LJ Saif, AR Kalica, HB Greenberg, AZ Kapikian, and RM Chanock

A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Group A Human Rotavirus Genomics: Evidence that Gene Constellations Are Influenced by Viral Protein Interactions.
E. M. Heiman, S. M. McDonald, M. Barro, Z. F. Taraporewala, T. Bar-Magen, and J. T. Patton (2008)
J. Virol. 82, 11106-11116
   Abstract »    Full Text »    PDF »
High Titers of Circulating Maternal Antibodies Suppress Effector and Memory B-Cell Responses Induced by an Attenuated Rotavirus Priming and Rotavirus-Like Particle-Immunostimulating Complex Boosting Vaccine Regimen.
T. V. Nguyen, L. Yuan, M. S. P. Azevedo, K.-i. Jeong, A. M. Gonzalez, C. Iosef, K. Lovgren-Bengtsson, B. Morein, P. Lewis, and L. J. Saif (2006)
Clin. Vaccine Immunol. 13, 475-485
   Abstract »    Full Text »    PDF »
Magnitude of Serum and Intestinal Antibody Responses Induced by Sequential Replicating and Nonreplicating Rotavirus Vaccines in Gnotobiotic Pigs and Correlation with Protection.
M. S. P. Azevedo, L. Yuan, C. Iosef, K.-O. Chang, Y. Kim, T. Van Nguyen, and L. J. Saif (2004)
Clin. Vaccine Immunol. 11, 12-20
   Abstract »    Full Text »    PDF »
Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with Mutant Escherichia coli Heat-Labile Toxin in Gnotobiotic Pigs.
L. Yuan, C. Iosef, M. S. P. Azevedo, Y. Kim, Y. Qian, A. Geyer, T. V. Nguyen, K.-O. Chang, and L. J. Saif (2001)
J. Virol. 75, 9229-9238
   Abstract »    Full Text »    PDF »
Intranasal Administration of 2/6-Rotavirus-Like Particles with Mutant Escherichia coli Heat-Labile Toxin (LT-R192G) Induces Antibody-Secreting Cell Responses but Not Protective Immunity in Gnotobiotic Pigs.
L. Yuan, A. Geyer, D. C. Hodgins, Z. Fan, Y. Qian, K.-O. Chang, S. E. Crawford, V. Parreño, L. A. Ward, M. K. Estes, et al. (2000)
J. Virol. 74, 8843-8853
   Abstract »    Full Text »
Isolation of a Human Rotavirus Strain with a Super-Short RNA Pattern and a New P2 Subtype.
T. Nakagomi, Y. Horie, Y. Koshimura, H. B. Greenberg, and O. Nakagomi (1999)
J. Clin. Microbiol. 37, 1213-1216
   Abstract »    Full Text »
Effects of Maternal Antibodies on Protection and Development of Antibody Responses to Human Rotavirus in Gnotobiotic Pigs.
D. C. Hodgins, S. Y. Kang, L. deArriba, V. Parreno, L. A. Ward, L. Yuan, T. To, and L. J. Saif (1999)
J. Virol. 73, 186-197
   Abstract »    Full Text »    PDF »
Analysis of Host Range Restriction Determinants in the Rabbit Model: Comparison of Homologous and Heterologous Rotavirus Infections.
M. Ciarlet, M. K. Estes, C. Barone, R. F. Ramig, and M. E. Conner (1998)
J. Virol. 72, 2341-2351
   Abstract »    Full Text »    PDF »
Antibody-Secreting Cell Responses and Protective Immunity Assessed in Gnotobiotic Pigs Inoculated Orally or Intramuscularly with Inactivated Human Rotavirus.
L. Yuan, S.-Y. Kang, L. A. Ward, T. L. To, and L. J. Saif (1998)
J. Virol. 72, 330-338
   Abstract »    Full Text »    PDF »
Rotavirus vaccines: success by reassortment?.
R. Glass, J Gentsch, and J. Smith (1994)
Science 265, 1389-1391
   PDF »


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