Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 4 January 1980:
Vol. 207. no. 4426, pp. 82 - 84
DOI: 10.1126/science.7350644
Prev | Table of Contents | Next

Articles

Science, Vol 207, Issue 4426, 82-84
Copyright © 1980 by American Association for the Advancement of Science

articles

Intraclonal variation in proliferative potential of human diploid fibroblasts: stochastic mechanism for cellular aging

Smith JR and RG Whitney

At several points during the growth of a clone of human embryonic lung fibroblasts in vitro, 100 to 200 cells were removed at random and the proliferative potential of each cell was determined. At each sample point, a wide variation in remaining population doubling ability was observed among the individual cells and the distributions of doubling potentials were distinctly bimodal. Furthermore, the two cells arising from a single mitosis differed in their ability to proliferate by as many as eight population doublings (256-fold in the number of cells produced). The results suggest that a stochastic process is responsible for determining the limited proliferative potential of human embryonic lung fibroblasts.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA damage in telomeres and mitochondria during cellular senescence: is there a connection?.
J. F. Passos, G. Saretzki, and T. von Zglinicki (2007)
Nucleic Acids Res. 35, 7505-7513
   Abstract »    Full Text »    PDF »
Structural stability and chromosome-specific telomere length is governed by cis-acting determinants in humans.
B. Britt-Compton, J. Rowson, M. Locke, I. Mackenzie, D. Kipling, and D. M. Baird (2006)
Hum. Mol. Genet. 15, 725-733
   Abstract »    Full Text »    PDF »
Early-Senescing Human Skin Fibroblasts Do Not Demonstrate Accelerated Telomere Shortening.
M. Ferenac, D. Polancec, M. Huzak, O. M. Pereira-Smith, and I. Rubelj (2005)
J. Gerontol. A Biol. Sci. Med. Sci. 60, 820-829
   Abstract »    Full Text »    PDF »
Stochastic Variation in Telomere Shortening Rate Causes Heterogeneity of Human Fibroblast Replicative Life Span.
C. Martin-Ruiz, G. Saretzki, J. Petrie, J. Ladhoff, J. Jeyapalan, W. Wei, J. Sedivy, and T. von Zglinicki (2004)
J. Biol. Chem. 279, 17826-17833
   Abstract »    Full Text »    PDF »
Author's Response to Commentary: Cell Senescence: An Evaluation of Replicative Senescence in Culture as a Model for Aging In Situ.
J. R. Smith (2003)
J. Gerontol. A Biol. Sci. Med. Sci. 58, B779-781
   Full Text »    PDF »
Modeling Stem Cell Population Growth: Incorporating Terms for Proliferative Heterogeneity.
B.M. Deasy, R.J. Jankowski, T.R. Payne, B. Cao, J.P. Goff, J.S. Greenberger, and J. Huard (2003)
Stem Cells 21, 536-545
   Abstract »    Full Text »    PDF »
Relationship between donor age and the replicative lifespan of human cells in culture: A reevaluation.
V. J. Cristofalo, R. G. Allen, R. J. Pignolo, B. G. Martin, and J. C. Beck (1998)
PNAS 95, 10614-10619
   Abstract »    Full Text »    PDF »
Aging and the cornea.
R G A FARAGHER, B MULHOLLAND, S J TUFT, S SANDEMAN, and P T KHAW (1997)
Br. J. Ophthalmol. 81, 814-817
   Full Text »    PDF »
Characterization of Fibroblast Clones from Periodontal Granulation Tissue in vitro.
L. Hakkinen and H. Larjava (1992)
Journal of Dental Research 71, 1901-1907
   Abstract »    PDF »
The Cultured Diploid Fibroblast as a Model for the Study of Cellular Aging.
T. H. Norwood and W. R. Pendergrass (1992)
Critical Reviews in Oral Biology & Medicine 3, 353-370
   Abstract »    Full Text »    PDF »
Drug-Induced Gingival Overgrowth: Old Problem, New Problem.
T. M. Hassell and A. F. Hefti (1991)
Critical Reviews in Oral Biology & Medicine 2, 103-137
   Full Text »    PDF »
Replicative senescence: the human fibroblast comes of age.
S Goldstein (1990)
Science 249, 1129-1133
   Abstract »    PDF »
Heterogeneity of normal human diploid fibroblasts: isolation and characterization of one phenotype.
S Bordin, R. Page, and A. Narayanan (1984)
Science 223, 171-173
   Abstract »    PDF »
Cellular aging: further evidence for the commitment theory.
R Holliday, L. Huschtscha, and T. Kirkwood (1981)
Science 213, 1505-1508
   Abstract »    PDF »
Ozone selectively inhibits growth of human cancer cells.
F Sweet, M. Kao, S. Lee, W. Hagar, and W. Sweet (1980)
Science 209, 931-933
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)