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Science 30 November 1979:
Vol. 206. no. 4422, pp. 1081 - 1083
DOI: 10.1126/science.227059
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Articles

Science, Vol 206, Issue 4422, 1081-1083
Copyright © 1979 by American Association for the Advancement of Science

articles

A sign inversion mechanism for enzymatic supercoiling of DNA

PO Brown and NR Cozzarelli

Both the introduction and the removal of supertwists by DNA gyrase change the linking number of DNA in steps of two. This surprising finding provides strong evidence that gyrase acts by a mechanism, called sign inversion, whereby a positive supercoil is directly inverted into a negative one via a transient double-strand break.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Unlocking and opening a DNA gate.
J. C. Wang (2007)
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Topoisomerase Action on Short DNA Duplexes Reveals Requirements for Gate and Transfer DNA Segments.
B. P. Belotserkovskii, P. B. Arimondo, and N. R. Cozzarelli (2006)
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Novel Symmetric and Asymmetric DNA Scission Determinants for Streptococcus pneumoniae Topoisomerase IV and Gyrase Are Clustered at the DNA Breakage Site.
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Coupling DNA Supercoiling to Transcription in Defined Protein Systems.
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The C-terminal domain of DNA gyrase A adopts a DNA-bending {beta}-pinwheel fold.
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Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance.
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From the Cover: The mechanism of type IA topoisomerases.
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ATPase Domain of Eukaryotic DNA Topoisomerase II. INHIBITION OF ATPase ACTIVITY BY THE ANTI-CANCER DRUG BISDIOXOPIPERAZINE AND ATP/ADP-INDUCED DIMERIZATION.
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Topological challenges to DNA replication: Conformations at the fork.
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PNAS 98, 8219-8226
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The Mechanism of Inhibition of Topoisomerase IV by Quinolone Antibacterials.
A. B. Khodursky and N. R. Cozzarelli (1998)
J. Biol. Chem. 273, 27668-27677
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Structural similarities between topoisomerases that cleave one or both DNA strands.
J. M. Berger, D. Fass, J. C. Wang, and S. C. Harrison (1998)
PNAS 95, 7876-7881
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A Model for the Mechanism of Human Topoisomerase I.
L. Stewart, M. R. Redinbo, X. Qiu, W. G. Hol, and J. J. Champoux (1998)
Science 279, 1534-1541
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The DNA Cleavage Reaction of DNA Gyrase. COMPARISON OF STABLE TERNARY COMPLEXES FORMED WITH ENOXACIN AND CcdB PROTEIN.
K. E. Scheirer and N. P. Higgins (1997)
J. Biol. Chem. 272, 27202-27209
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Contrasting Enzymatic Activities of Topoisomerase IV and DNA Gyrase from Escherichia coli.
C. Ullsperger and N. R. Cozzarelli (1996)
J. Biol. Chem. 271, 31549-31555
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Escherichia coli DNA Topoisomerase III Is a Site-specific DNA Binding Protein That Binds Asymmetrically to Its Cleavage Site.
H. L. Zhang, S. Malpure, and R. J. DiGate (1995)
J. Biol. Chem. 270, 23700-23705
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Biochemical topology: applications to DNA recombination and replication.
S. Wasserman and N. Cozzarelli (1986)
Science 232, 951-960
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A Topological Treatment of Recombination and Topoisomerases.
N.R. Cozzarelli, M.A. Krasnow, S.P. Gerrard, and J.H. White (1984)
Cold Spring Harb Symp Quant Biol 49, 383-400
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Escherichia coli Type-1 Topoisomerases: Identification, Mechanism, and Role in Recombination.
F. Dean, M.A. Krasnow, R. Otter, M.M. Matzuk, S.J. Spengler, and N.R. Cozzarelli (1983)
Cold Spring Harb Symp Quant Biol 47, 769-777
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DNA Gyrase: Site-specific Interactions and Transient Double-strand Breakage of DNA.
M. Gellert, L. M. Fisher, H. Ohmori, M. H. O'Dea, and K. Mizuuchi (1981)
Cold Spring Harb Symp Quant Biol 45, 391-398
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In Vitro Study of Illegitimate Recombination: Involvement of DNA Gyrase.
H. Ikeda, K. Moriya, and T. Matsumoto (1981)
Cold Spring Harb Symp Quant Biol 45, 399-408
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Strand Exchange in {lambda} Integrative Recombination: Genetics, Biochemistry, and Models.
H. A. Nash, K. Mizuuchi, L. W. Enquist, and R. A. Weisberg (1981)
Cold Spring Harb Symp Quant Biol 45, 417-428
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DNA gyrase and the supercoiling of DNA.
N. Cozzarelli (1980)
Science 207, 953-960
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