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Science 30 November 1979:
Vol. 206. no. 4422, pp. 1077 - 1079
DOI: 10.1126/science.227058
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Articles

Science, Vol 206, Issue 4422, 1077-1079
Copyright © 1979 by American Association for the Advancement of Science

articles

Opiate (Enkephalin) receptors of neuroblastoma cells: occurrence in clusters on the cell surface

E Hazum, KJ Chang, and P Cuatrecasas

A bioactive, fluorescent derivative of enkephalin, Tyr-D-Ala-Gly-Phe-Leu-Lys-rhodamine, was used to determine the distribution of opiate receptors in living neuroblastoma cells. The receptors appeared in clusters on the cell surface, and no internalization was detected. No specific fluorescence or clusters were observed in the presence of [D-Ala2, Leu5]enkephalin or at 4 degrees C, and the clusters were much reduced under ionic conditions (that is, with 100 millimolars sodium) that specifically decrease the binding of opiate agonists.


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Visualization of specific angiotensin II binding sites in the brain by fluorescent microscopy.
S Landas, M. Phillips, J. Stamler, and M. Raizada (1980)
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