Science, Vol 206, Issue 4415, 220-222
Copyright © 1979 by American Association for the Advancement of Science
Expression of the Escherichia coli cell division gene sep cloned in a lambda Charon phage
CA Irwin,
G Fletcher,
CL Sills,
and
Walker JR
The Escherichia coli cell division gene sep, which probably codes for one of the penicillin-binding proteins, has been cloned into lambda Charon 10 to form a viable sep+ transducing phage. After infection with this hybrid phage, penicillin-binding protein 3 was overproduced and incorporated into the E. coli inner membrane.