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Science 7 September 1979:
Vol. 205. no. 4410, pp. 1007 - 1010
DOI: 10.1126/science.382356
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Articles

Science, Vol 205, Issue 4410, 1007-1010
Copyright © 1979 by American Association for the Advancement of Science

articles

Genetic effects of impure and pure saccharin in yeast

CW Moore and A Schmick

Yeast cells were grown in media containing impure or purified saccharin preparations. Dose-dependent increases in frequencies of cells possessing aberrant cell morphologies were revealed by light microscopy. At each test dose, cells grown in impure saccharin exhibited up to sevenfold higher frequencies of mitotic crossing-over or gene conversion in three of four assays for genetic recombination than cells grown in purified saccharin from the same lot. With one exception, the sweetener produced by the Maumee process caused larger increases in recombination and gene reversion than the sweetener produced by the Remsen-Fahlberg process. The several test markers did not respond equally to any test saccharin. Cells grown in liquid media containing no saccharin or two of three test concentrations of saccharin produced cell titers that were approximately equivalent.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA Damage-Inducible and RAD52-Independent Repair of DNA Double-Strand Breaks in Saccharomyces cerevisiae.
C. W. Moore, J. McKoy, M. Dardalhon, D. Davermann, M. Martinez, and D. Averbeck (2000)
Genetics 154, 1085-1099
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