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Science 24 August 1979:
Vol. 205. no. 4408, pp. 821 - 823
DOI: 10.1126/science.462192
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Articles

Science, Vol 205, Issue 4408, 821-823
Copyright © 1979 by American Association for the Advancement of Science

articles

GABA receptors in clonal cell lines: a model for study of benzodiazepine action at molecular level

M Baraldi, A Guidotti, JP Schwartz, and E Costa

A "recptor unit" for gamma-aminobutyric acid (GABA), which includes brainlike receptor binding sites for tritium-labeled GABA and benzodiazepines (diazepam, clonazepam, and flunitrazepam) and a thermostable endogenous protein (GABA modulin) that inhibits both GABA and benzodiazepine binding, has been demonstrated in membranes prepared from NB2a neuroblastoma and C6 glioma clonal cell lines. In these cells, as in brain, diazepam (1 micromolar) prevents the effect of GABA modulin, and in turn GABA (0.oma and, to a lesser extent, the glioma cells represent a suitable model to study the interactions and the sequence of membrane and intracellular events triggered by the stimulation of benzodiazepine and GABA receptors.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Isolation and Characterization of an Endogenous Peptide from Rat Brain Interacting Specifically with the Serotonergic 1B Receptor Subtypes.
J.-C. Rousselle, O. Massot, M. Delepierre, E. Zifa, B. Rousseau, and G. Fillion (1996)
J. Biol. Chem. 271, 726-735
   Abstract »    Full Text »    PDF »
Experimental hepatic encephalopathy: changes in the binding of gamma-aminobutyric acid.
M Baraldi and Z. Zeneroli (1982)
Science 216, 427-429
   Abstract »    PDF »


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