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Science 17 August 1979:
Vol. 205. no. 4407, pp. 691 - 693
DOI: 10.1126/science.223242
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Articles

Science, Vol 205, Issue 4407, 691-693
Copyright © 1979 by American Association for the Advancement of Science

articles

Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen

HY Cho and JS Rhim

The protein synthesis inhibitor cycloheximide, at a concentration of 0.08 microgram per milliliter, induced flat morphology within 24 to 48 hours and low saturation density in human osteosarcoma cells transformed by Kirsten murine sarcoma virus (Ki-MSV) or N-methyl-N' nitro-N-nitrosoguanidine. Removal of the protein synthesis inhibitor caused both transformed cells to revert to the transformed phenotype. The demonstration of cell-surface antigens, cross-reacted with antiserums induced by extracts of both types of transformed human cells, was dependent on the presence or absence of cycloheximide in the culture medium. The results show that protein synthesis is required to maintain the transformed state in virally or chemically transformed human cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mode of Transmission of Legionella pneumophila: A Critical Review.
R. R. Muder, V. L. Yu, and A. H. Woo (1986)
Arch Intern Med 146, 1607-1612
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