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Science 17 August 1979:
Vol. 205. no. 4407, pp. 688 - 689
DOI: 10.1126/science.205.4407.688
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Articles

Cell-Free Nitrogenase and Hydrogenase from Actinorhizal Root Nodules

DAVID R. BENSON 1, DANIEL J. ARP 1, and R. H. BURRIS 1

1 Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison 53706

Field-grown alder (Alnus glutinosa) root nodules were disrupted in liquid nitrogen to release the actinomycete endophytes. The endophytes were broken by mild sonic oscillation and yielded a cell-free nitrogenase preparation capable of reducing acetylene and protons. In addition, the preparation carried a cell-free uptake hydrogenase.

Submitted on February 27, 1979
Revised on May 15, 1979

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Science. ISSN 0036-8075 (print), 1095-9203 (online)