Related Content
Search Google Scholar for:
|
|
Science 13 July 1979:
Vol. 205. no. 4402, pp. 160 - 166
DOI: 10.1126/science.36664
|
|
Articles
Science, Vol 205, Issue 4402, 160-166
Copyright © 1979 by American Association for the Advancement of Science
Cellular applications of 31P and 13C nuclear magnetic resonance
RG Shulman,
TR Brown,
K Ugurbil,
S Ogawa,
SM Cohen,
and
JA den Hollander
High-resolution nuclear magnetic resonance (NMR) studies of cells and purified mitochondria are discussed to show the kind of information that can be obtained in vivo. In suspensions of Escherichia coli both phosphorus-31 and carbon-13 NMR studies of glycolysis and bioenergetics are presented. In rat liver cells the pathways of gluconeogenesis from carbon-13-labeled glycerol are followed by carbon-13 NMR. In the intact liver cells cytosolic and mitochondrial pH's were separately measured by phosphorus-31 NMR. In purified mitochondria the internal and external concentrations of inorganic phosphate, adenosine diphosphate, and adenosine triphosphate were determined by phosphorus-31 NMR while the pH difference across the membrane was measured simultaneously.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB2 ABC Transporter in Escherichia coli.
- C. D. Rice, J. E. Pollard, Z. T. Lewis, and W. R. McCleary (2009)
Appl. Envir. Microbiol.
75, 573-582
| Abstract »
| Full Text »
| PDF »
- Beyond aerobic glycolysis: Transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis.
- R. J. DeBerardinis, A. Mancuso, E. Daikhin, I. Nissim, M. Yudkoff, S. Wehrli, and C. B. Thompson (2007)
PNAS
104, 19345-19350
| Abstract »
| Full Text »
| PDF »
- Kinetic Characterization of the ATPase Cycle of the Molecular Chaperone Hsc66 from Escherichia coli.
- J. J. Silberg and L. E. Vickery (2000)
J. Biol. Chem.
275, 7779-7786
| Abstract »
| Full Text »
| PDF »
- In Vivo Fluxes in the Ammonium-Assimilatory Pathways in Corynebacterium glutamicum Studied by 15N Nuclear Magnetic Resonance.
- M. Tesch, A. A. de Graaf, and H. Sahm (1999)
Appl. Envir. Microbiol.
65, 1099-1109
| Abstract »
| Full Text »
- Defective Export of a Periplasmic Enzyme Disrupts Regulation of Fatty Acid Synthesis.
- H. Cho and J. E. Cronan Jr. (1995)
J. Biol. Chem.
270, 4216-4219
| Abstract »
| Full Text »
| PDF »
- Magnetic Resonance Spectroscopy: A Technique for Functional Brain Imaging.
- B. H. Guze (1991)
Arch Gen Psychiatry
48, 572-574
| Abstract »
| PDF »
- Nuclear magnetic resonance technology for medical studies.
- T. Budinger and P. Lauterbur (1984)
Science
226, 288-298
| Abstract »
| PDF »
- In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy.
- C. Unkefer, R. Blazer, and R. London (1983)
Science
222, 62-65
| Abstract »
| PDF »
- Nuclear Magnetic Resonance.
- H. A. BALTAXE and M. C. GEOKAS (1983)
Ann Intern Med
98, 540-542
| Abstract »
| PDF »
- Proton nuclear magnetic resonance of intact Friend leukemia cells: phosphorylcholine increase during differentiation.
- P. Agris and I. Campbell (1982)
Science
216, 1325-1327
| Abstract »
| PDF »
- In vivo carbon-13 nuclear magnetic resonance studies of mammals.
- Alger JR, L. Sillerud, K. Behar, R. Gillies, R. Shulman, R. Gordon, D Shae, and P. Hanley (1981)
Science
214, 660-662
| Abstract »
| PDF »
- Assessment of pharmacological treatment of myocardial infarction by phosphorus-31 NMR with surface coils.
- R. Nunnally and P. Bottomley (1981)
Science
211, 177-180
| Abstract »
| PDF »
- Polynucleotide phosphorylase functions both as a 3' right-arrow 5' exonuclease and a poly(A) polymerase in Escherichiacoli.
- B. K. Mohanty and S. R. Kushner (2000)
PNAS
97, 11966-11971
| Abstract »
| Full Text »
| PDF »
|
|
