Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 22 June 1979:
Vol. 204. no. 4399, pp. 1311 - 1313
DOI: 10.1126/science.451538
Prev | Table of Contents | Next

Articles

Science, Vol 204, Issue 4399, 1311-1313
Copyright © 1979 by American Association for the Advancement of Science

articles

Evidence for extra-renal 1 alpha-hydroxylation of 25-hydroxyvitamin D3 in pregnancy

TK Gray, GE Lester, and RS Lorenc

The kidneys are thought to be the only organs capable of 1 alpha-hydroxylation of vitamin D and its metabolites. We have examined the in vivo conversion of 3H-(25,26)-25-hydroxyvitamin D3(25OHD3) to 3H-(25,26)-1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] in vitamin D-deficient, pregnant and nonpregnant rats. As expected, nephrectomy of nonpregnant, vitamin D-deficient rats prevented the conversion of 25OHD3 to 1 alpha,25(OH)2D3. In contrast, nephrectomy of pregnant, vitamin D-deficient rats reduced but did not abolish the formation of 1 alpha,25(OH)2D3 from its precursor. The identity of the radioactive metabolite formed from 3H-25OHD3 which circulated in nephrectomized, pregnant rats was established as 1 alpha,25(OH)2D3 by comigration with synthetic 1 alpha,25(OH)2D3 on high-pressure liquid chromatography. The simultaneous absence of 1 alpha,25(OH)2D3 in the fetal kidneys indicated that the site of 1 alpha-hydroxylation after nephrectomy of the pregnant rat was probably extra-renal in origin. Two sites of 1 alpha-hydroxylation of 25OHD3, one renal and the other extra-renal, either fetoplacental or maternal, may exist in the pregnant, vitamin D-deficient rat.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Vitamin D Induces Innate Antibacterial Responses in Human Trophoblasts via an Intracrine Pathway.
N. Liu, A.T. Kaplan, J. Low, L. Nguyen, G.Y. Liu, O. Equils, and M. Hewison (2009)
Biol Reprod 80, 398-406
   Abstract »    Full Text »    PDF »
Respiratory Epithelial Cells Convert Inactive Vitamin D to Its Active Form: Potential Effects on Host Defense.
S. Hansdottir, M. M. Monick, S. L. Hinde, N. Lovan, D. C. Look, and G. W. Hunninghake (2008)
J. Immunol. 181, 7090-7099
   Abstract »    Full Text »    PDF »
Effects of 25-Hydroxyvitamin D3 and 1,25-Dihydroxyvitamin D3 on Cytokine Production by Human Decidual Cells.
K. N. Evans, L. Nguyen, J. Chan, B. A. Innes, J. N. Bulmer, M. D. Kilby, and M. Hewison (2006)
Biol Reprod 75, 816-822
   Abstract »    Full Text »    PDF »
CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D3-1{alpha}-hydroxylase promoter activity in the skin.
J. L. Vanhooke, J. M. Prahl, C. Kimmel-Jehan, M. Mendelsohn, E. W. Danielson, K. D. Healy, and H. F. DeLuca (2006)
PNAS 103, 75-80
   Abstract »    Full Text »    PDF »
Vitamin D and Placental-Decidual Function.
K. N. Evans, J. N. Bulmer, M. D. Kilby, and M. Hewison (2004)
Reproductive Sciences 11, 263-271
   Abstract »    PDF »
The Ontogeny of 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase Expression in Human Placenta and Decidua.
D. Zehnder, K. N. Evans, M. D. Kilby, J. N. Bulmer, B. A. Innes, P. M. Stewart, and M. Hewison (2002)
Am. J. Pathol. 161, 105-114
   Abstract »    Full Text »    PDF »
Extrarenal Expression of 25-Hydroxyvitamin D3-1{{alpha}}-Hydroxylase.
D. Zehnder, R. Bland, M. C. Williams, R. W. McNinch, A. J. Howie, P. M. Stewart, and M. Hewison (2001)
J. Clin. Endocrinol. Metab. 86, 888-894
   Abstract »    Full Text »
Calcium metabolism in sarcoidosis and its clinical implications.
M. Conron, C. Young, and H. L. C. Beynon (2000)
Rheumatology 39, 707-713
   Abstract »    Full Text »    PDF »
Maternal-Fetal Calcium and Bone Metabolism During Pregnancy, Puerperium, and Lactation.
C. S. Kovacs and H. M. Kronenberg (1997)
Endocr. Rev. 18, 832-872
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)