Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 25 May 1979:
Vol. 204. no. 4395, pp. 865 - 866
DOI: 10.1126/science.441741
Prev | Table of Contents | Next

Articles

Science, Vol 204, Issue 4395, 865-866
Copyright © 1979 by American Association for the Advancement of Science

articles

Immunoassay by differential pulse polarography

WR Heineman, CW Anderson, and HB Halsall

An immunological method based on labeling an antigen with an electroactive group detectable by differential pulse polarography has been demonstrated. Estriol labeled with mercuric acetate is electroactive, giving a reduction wave at -300 millivolts versus a standard calomel electrode. Addition of estriol antibody to 4-mercuric acetate estriol diminishes the peak current as a result of the antigen-antibody binding reaction. Separation of free-labeled estriol from antibody-bound-labeled antigen is unnecessary. The method is potentially useful as an analytical immunological technique.




To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)