Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 25 May 1979:
Vol. 204. no. 4395, pp. 862 - 865
DOI: 10.1126/science.35828
Prev | Table of Contents | Next

Articles

Science, Vol 204, Issue 4395, 862-865
Copyright © 1979 by American Association for the Advancement of Science

articles

Intercellular communication in pancreatic islet monolayer cultures: a microfluorometric study

E Kohen, C Kohen, B Thorell, DH Mintz, and A Rabinovitch

Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry. Fluorescein spread from an injected islet cell directly into neighboring islet cells, and, in the presence of 16.7 millimolar glucose, significantly more islet cells communicated with the injected cell than in glucose-free medium. Islet cells were also microinjected with glycolytic substrates and activators that produced transient changes in cellular levels of reduced pyridine nucleotides-nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate [NAD(P)H]. Changes in NAD(P)H fluorescence were observed in islet cells incubated first for 18 hours in very low glucose concentrations and then in a glucose-free medium and injected with glycolytic substrates and activators; however, little change of fluorescence occurred in adjacent islet cells. In contrast, after adding 16.7 millimolar glucose to the medium, injection of glycolytic substrates and activators produced transient changes in NAD(P)H fluorescence in the injected cell and in neighboring cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
On-line analysis of gap junctions reveals more efficient electrical than dye coupling between islet cells.
I. Quesada, E. Fuentes, E. Andreu, P. Meda, A. Nadal, and B. Soria (2003)
Am J Physiol Endocrinol Metab 284, E980-E987
   Abstract »    Full Text »    PDF »
Hyperinsulinemia-Induced Hypoglycemia Is Enhanced by Overexpression of Connexin 43.
C. Vozzi, D. Bosco, E. Dupont, A. Charollais, and P. Meda (1997)
Endocrinology 138, 2879-2885
   Abstract »    Full Text »    PDF »
A fluorescence photobleaching assay of gap junction-mediated communication between human cells.
M. Wade, J. Trosko, and M Schindler (1986)
Science 232, 525-528
   Abstract »    PDF »
Islets of Langerhans: dye coupling among immunocytochemically distinct cell types.
R. Michaels and J. Sheridan (1981)
Science 214, 801-803
   Abstract »    PDF »
Gap junction development is correlated with insulin content in the pancreatic B cell.
P Meda, P Halban, A Perrelet, A. Renold, and L Orci (1980)
Science 209, 1026-1028
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)