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Science 27 April 1979:
Vol. 204. no. 4391, pp. 403 - 404
DOI: 10.1126/science.441727
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Articles

Science, Vol 204, Issue 4391, 403-404
Copyright © 1979 by American Association for the Advancement of Science

articles

Flow cytometry: a high-resolution instrument for everyone

HB Steen and T Lindmo

A new flow configuration for flow cytometry has been devised in which a flat, laminar stream of water, containing the stained cells in a narrow sector, is formed on a microscope cover slip by a pressurized jet of water directed onto the glass at low angle. The stream of cells is viewed by means of a fluorescence microscope with incident illumination and a pulse photometer. Coupled to a multichannel pulse height analyzer, the instrument constitutes a stable and easy-to-operate flow cytometer with a resolution equal to or better than a coefficient of variance of 1.4 percent in measurements of cellular DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Effects of the Metalloid Oxyanion Tellurite (TeO32-) on Growth Characteristics of the Phototrophic Bacterium Rhodobacter capsulatus.
R. Borghese, F. Borsetti, P. Foladori, G. Ziglio, and D. Zannoni (2004)
Appl. Envir. Microbiol. 70, 6595-6602
   Abstract »    Full Text »    PDF »
Time: a new parameter for kinetic measurements in flow cytometry.
J. Martin and D. Swartzendruber (1980)
Science 207, 199-201
   Abstract »    PDF »


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