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Science 27 April 1979:
Vol. 204. no. 4391, pp. 375 - 380
DOI: 10.1126/science.220706
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Articles

Science, Vol 204, Issue 4391, 375-380
Copyright © 1979 by American Association for the Advancement of Science

articles

Space-filling models of kinase clefts and conformation changes

CM Anderson, FH Zucker, and TA Steitz

Space-filling models of yeast hexokinase, adenylate kinase, and phosphoglycerate kinase drawn by computer clearly portray the bilobal character of these phosphoryl transfer enzymes, and the deep cleft which is formed between the lobes. A dramatic conformational change occurs in hexokinase as glucose binds to the bottom of the cleft, which causes the two lobes of hexokinase to come together. A substrate-induced closing of the active site cleft is postulated to occur in other kinases as well. This change may provide a mechanism by which some of these enzymes reduce their inherent adenosine triphosphatase activity and could be a general requirement of the kinase reaction.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)