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Science 13 April 1979:
Vol. 204. no. 4389, pp. 176 - 177
DOI: 10.1126/science.432639
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Articles

Science, Vol 204, Issue 4389, 176-177
Copyright © 1979 by American Association for the Advancement of Science

articles

Dexamethasone stimulation of metallothionein synthesis in HeLa cell cultures

M Karin and HR Herschman

HeLa cells in culture synthesize metallothionein. To investigate the effects of glucocorticoids on metallothionein synthesis we adapted HeLa cells to growth in a defined medium lacking hydrocortisone. In this defined medium, containing 1.5 x 10(-6)M Zn2+, dexamethasone (10(-6)M) caused a five- to sixfold increase in the synthesis of metallothionein. Progesterone (10(-5)M), a known antagonist of glucocorticoids, inhibited this response by 50 percent.


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Clin. Cancer Res. 11, 6325-6332
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High-glucose-induced metallothionein expression in endothelial cells: an endothelin-mediated mechanism.
M. D. Apostolova, S. Chen, S. Chakrabarti, and M. G. Cherian (2001)
Am J Physiol Cell Physiol 281, C899-C907
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trans Repression of the Human Metallothionein IIA Gene Promoter by PZ120, a Novel 120-Kilodalton Zinc Finger Protein.
C.-M. Tang, J. Westling, and E. Seto (1999)
Mol. Cell. Biol. 19, 680-689
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Covalent Sequestration of the Nitrogen Mustard Mechlorethamine by Metallothionein.
M. Antoine, D. Fabris, and C. Fenselau (1998)
Drug Metab. Dispos. 26, 921-926
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A pair of adjacent glucocorticoid response elements regulate expression of two mouse metallothionein genes.
E. J. Kelly, E. P. Sandgren, R. L. Brinster, and R. D. Palmiter (1997)
PNAS 94, 10045-10050
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Excitation-Transcription Coupling Mediated by Zinc Influx through Voltage-dependent Calcium Channels.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)