Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 23 March 1979:
Vol. 203. no. 4386, pp. 1251 - 1253
DOI: 10.1126/science.424752
Prev | Table of Contents | Next

Articles

Science, Vol 203, Issue 4386, 1251-1253
Copyright © 1979 by American Association for the Advancement of Science

articles

Premature senescence in cultured skin fibroblasts from subjects with cystic fibrosis

BL Shapiro, LF Lam, and LH Fast

Cultured skin fibroblasts from subjects with cystic fibrosis exhibited normal population doubling times in early passages. After about 13 cumulative population doublings, cystic fibrosis lines doubled more slowly than controls and ceased doubling after about 19 weekly passages. Control lines continued doubling for 27 passages. The premature senescence noted in cells from subjects with cystic fibrosis reconciles controversial observations of cell doubling reported in the literature. Data presented here demonstrate that experiments with cystic fibrosis cells in late passage may generate misleading results since differences from control lines may be ascribed to generalized senile changes rather than to specific results of the cystic fibrosis genotype.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Calcium and age in fibroblasts from control subjects and patients with cystic fibrosis.
B. Shapiro and L. Lam (1982)
Science 216, 417-419
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)