Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 March 1979:
Vol. 203. no. 4384, pp. 1019 - 1021
DOI: 10.1126/science.424726
Prev | Table of Contents | Next

Articles

Science, Vol 203, Issue 4384, 1019-1021
Copyright © 1979 by American Association for the Advancement of Science

articles

Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II

J Singer, J Roberts-Ems, and AD Riggs

The restriction enzymes Hpa II and Msp I both recognize the sequence 5'-CCGG (C, cytosine; G, guanine). However, Hpa II cuts mouse liver DNA to fragments four times larger than does Msp I. The size of DNA cut by Msp I is close to that predicted from base composition and nearest neighbor analysis. The most probable explanation of these results is that in mouse the site 5'-CCGG is highly methylated.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inaugural Article: A human B cell methylome at 100-base pair resolution.
T. A. Rauch, X. Wu, X. Zhong, A. D. Riggs, and G. P. Pfeifer (2009)
PNAS 106, 671-678
   Abstract »    Full Text »    PDF »
Comparative isoschizomer profiling of cytosine methylation: The HELP assay.
B. Khulan, R. F. Thompson, K. Ye, M. J. Fazzari, M. Suzuki, E. Stasiek, M. E. Figueroa, J. L. Glass, Q. Chen, C. Montagna, et al. (2006)
Genome Res. 16, 1046-1055
   Abstract »    Full Text »    PDF »
Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation.
S. Yegnasubramanian, X. Lin, M. C. Haffner, A. M. DeMarzo, and W. G. Nelson (2006)
Nucleic Acids Res. 34, e19
   Abstract »    Full Text »    PDF »
Down-regulation of candidate tumor suppressor genes within chromosome band 13q14.3 is independent of the DNA methylation pattern in B-cell chronic lymphocytic leukemia.
D. Mertens, S. Wolf, P. Schroeter, C. Schaffner, H. Dohner, S. Stilgenbauer, and P. Lichter (2002)
Blood 99, 4116-4121
   Abstract »    Full Text »    PDF »
Methylation of the minimal promoter of an embryonic globin gene silences transcription in primary erythroid cells.
R. Singal, R. Ferris, J. A. Little, S. Z. Wang, and G. D. Ginder (1997)
PNAS 94, 13724-13729
   Abstract »    Full Text »    PDF »
Characterization of the Transcription Unit of Mouse Kv1.4, a Voltage-gated Potassium Channel Gene.
R. S. Wymore, D. Negulescu, K. Kinoshita, K. Kalman, J. Aiyar, G. A. Gutman, and K. G. Chandy (1996)
J. Biol. Chem. 271, 15629-15634
   Abstract »    Full Text »    PDF »
Application of Synthetic DNA Probes to the Analysis of DNA Sequence Variants in Man.
R.B. Wallace, L.D. Petz, and P.Y. Yam (1986)
Cold Spring Harb Symp Quant Biol 51, 257-261
   Abstract »    PDF »
5-Methylcytosine in eukaryotic DNA.
M Ehrlich and R. Wang (1981)
Science 212, 1350-1357
   Abstract »    PDF »
DNA methylation and gene function.
A Razin and A. Riggs (1980)
Science 210, 604-610
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)