Science, Vol 203, Issue 4384, 1016-1019
Copyright © 1979 by American Association for the Advancement of Science
Regional localization of the gene for human phosphoribosylpyrophosphate synthetase on the X chromosome
MA Becker,
RC Yen,
P Itkin,
SJ Goss,
JE Seegmiller,
and
B Bakay
Sixty-eight independent hybrid clones were isolated after irradiated normal human lymphocytes were fused with Chinese hamster fibroblasts lacking hypoxanthine-guanine phosphoribosyltransferase activity. The cells were grown under selective conditions requiring retention of the X chromosome-linked locus for human hypoxanthine-guanine phosphoribosyltransferase. The frequency and patterns of cotransference of human phosphoribosylpyrophosphate synthetase with the selected marker and with additional X-linked enzymatic markers confirm X linkage of the structural gene for human phosphoribosylpyrophosphate synthetase and support assignment of this gene to a position on the long arm of the X, between the loci for alpha-galactosidase and hypoxanthine-guanine phosphoribosyltransferase.
