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Science 22 December 1978:
Vol. 202. no. 4374, pp. 1295 - 1298
DOI: 10.1126/science.725606
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Articles

Science, Vol 202, Issue 4374, 1295-1298
Copyright © 1978 by American Association for the Advancement of Science

articles

Cell lineage analysis by intracellular injection of a tracer enzyme

DA Weisblat, RT Sawyer, and GS Stent

Cell lineages during development of leeches can be ascertained by injection of horseradish peroxidase as a tracer into identified cells at early stages of embryogenesis. The injected embryos continue their normal development, in the course of which horseradish peroxidase is passed on in catalytically active form to the descendants of the injected cell. The distribution of the tracer enzyme and hence of the progeny of the injected cell can then be observed at a later stage of development by staining the preparation for horseradish peroxidase.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Helobdella (Leech): A Model for Developmental Studies.
D. A. Weisblat and D.-H. Kuo (2009)
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Induction into the Hall of Fame: tracing the lineage of Spemann's organizer.
R. Harland (2008)
Development 135, 3321-3323
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Cell interactions that affect axonogenesis in the leech Theromyzon rude.
D. H. Shain, D. K. Stuart, F. Z. Huang, and D. A. Weisblat (2004)
Development 131, 4143-4153
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Cell lineage in marine nematode Enoplus brevis.
D. Voronov and Y. Panchin (1998)
Development 125, 143-150
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Unequal cleavage in leech embryos: zygotic transcription is required for correct spindle orientation in subset of early blastomeres.
S. Bissen and C. Smith (1996)
Development 122, 599-606
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Magnetic resonance microscopy of embryonic cell lineages and movements.
R. Jacobs and S. Fraser (1994)
Science 263, 681-684
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Lox10, a member of the NK-2 homeobox gene class, is expressed in a segmental pattern in the endoderm and in the cephalic nervous system of the leech Helobdella.
D Nardelli-Haefliger and M Shankland (1993)
Development 118, 877-892
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Lox2, a putative leech segment identity gene, is expressed in the same segmental domain in different stem cell lineages.
D Nardelli-Haefliger and M Shankland (1992)
Development 116, 697-710
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Germ Plasm and Germ Cell Determination in Xenopus laevis As Studied by Cell Transplantation Analysis.
C.C. Wylie, S. Holwill, M. O'driscoll, A. Snape, and J. Heasman (1985)
Cold Spring Harb Symp Quant Biol 50, 37-43
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Cell recognition during neuronal development.
C. Goodman, M. Bastiani, C. Doe, S du Lac, S. Helfand, J. Kuwada, and J. Thomas (1984)
Science 225, 1271-1279
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Cell lineage analysis by intracellular injection of fluorescent tracers.
D. Weisblat, S. Zackson, S. Blair, and J. Young (1980)
Science 209, 1538-1541
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Science. ISSN 0036-8075 (print), 1095-9203 (online)