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Science 22 December 1978:
Vol. 202. no. 4374, pp. 1279 - 1284
DOI: 10.1126/science.725603
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Articles

Science, Vol 202, Issue 4374, 1279-1284
Copyright © 1978 by American Association for the Advancement of Science

articles

Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections

FR Blattner, AE Blechl, K Denniston-Thompson, HE Faber, JE Richards, JL Slightom, PW Tucker, and O Smithies

Shotgun collections of Charon 3A bacteriophages containing Eco RI fragments of human and mouse DNA were constructed with the use of in vitro packaging. Plaques were screened by hybridization, and globin-specific clones were isolated from both human (Charon 3AHs51.1) and mouse (Charon 3AMm30.5). The fragments cloned were detected in unfractionated genomic DNA by the Southern method of hybridization.


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