Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 17 November 1978:
Vol. 202. no. 4369, pp. 760 - 763
DOI: 10.1126/science.715440
Prev | Table of Contents | Next

Articles

Science, Vol 202, Issue 4369, 760-763
Copyright © 1978 by American Association for the Advancement of Science

articles

Entry of insulin into human cultured lymphocytes: electron microscope autoradiographic analysis

ID Goldfine, AL Jones, GT Hradek, KY Wong, and JS Mooney

Electron microscope autoradiographs were prepared of IM-9 human cultured lymphocytes incubated with iodine-125-labeled insulin. With the use of [125I]insulin and Ilford L-4 emulsion, the technique had a resolution half-distance of approximately 0.085 micrometer. Autoradiographs revealed a time-dependent entry of insulin into the cell interior that was maximal after 30 minutes of incubation. At this time point nearly 40 percent of the [125I]insulin was in the interior of the cell at a distance 1 micrometer or greater from the plasma membrane. Grain distribution and volume density analyses revealed that the intracellular insulin was concentrated in the endoplasmic reticulum and nuclear membrane.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stimulation of RNA and protein synthesis by intracellular insulin.
D. Miller (1988)
Science 240, 506-509
   Abstract »    PDF »
Insulin stimulation of nucleoside triphosphatase activity in isolated nuclear envelopes.
F Purrello, R Vigneri, G. Clawson, and I. Goldfine (1982)
Science 216, 1005-1007
   Abstract »    PDF »
Evidence for a vesicular transport mechanism in hepatocytes for biliary secretion of immunoglobulin A.
R. Renston, A. Jones, W. Christiansen, G. Hradek, and B. Underdown (1980)
Science 208, 1276-1278
   Abstract »    PDF »
Principles of Membrane Receptor Physiology and Their Application to Clinical Medicine.
R. J. POLLET and G. S. LEVEY (1980)
Ann Intern Med 92, 663-680
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)