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Science 21 July 1978:
Vol. 201. no. 4352, pp. 259 - 262
DOI: 10.1126/science.208150
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Articles

Science, Vol 201, Issue 4352, 259-262
Copyright © 1978 by American Association for the Advancement of Science

articles

Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species

RH Yolken, B Barbour, RG Wyatt, AR Kalica, AZ Kapikian, and RM Chanock

Rotaviruses cause gastroenteritis in man and a wide variety of animal species. They cross-react in many immunologic tests and have a similar appearance by electron microscopy, making differentiation among them difficult. Rotaviruses derived from different host species were distinguished by postinfection serum blocking virus activity in an enzyme-linked immunosorbent assay (ELISA). Thirty-three rotavirus isolates from children living in three different parts of the world could not be differentiated by this technique, but they were distinct from four strains recovered from calves, and a series of strains isolated from piglets, foals, monkeys, and infant mice. The four bovine strains were similar, but they could be differentiated from the other animal strains, each of which exhibited a distinct pattern when tested by the ELISA blocking technique.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Bicozamycin, A Poorly Absorbable Antibiotic, Effectively Treats Travelers' Diarrhea.
C. D. ERICSSON, H. L. DuPONT, P. SULLIVAN, E. GALINDO, D. G. EVANS, and D. J. EVANS Jr. (1983)
Ann Intern Med 98, 20-25
   Abstract »    PDF »
Rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced by bovine virus.
R. Wyatt, C. Mebus, R. Yolken, A. Kalica, H. James Jr, A. Kapikian, and R. Chanock (1979)
Science 203, 548-550
   Abstract »    PDF »


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