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Science 23 June 1978:
Vol. 200. no. 4348, pp. 1388 - 1391
DOI: 10.1126/science.566465
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Articles

Science, Vol 200, Issue 4348, 1388-1391
Copyright © 1978 by American Association for the Advancement of Science

articles

Preparation of sarcolemmal membrane from myocardial tissue culture monolayer by high-velocity gas dissection

GA Langer, JS Frank, and KD Philipson

A high-velocity stream of nitrogen is used to simultaneously disrupt myocardial cells in monolayer culture and fractionate their sarcolemmal membranes. The membranes show a high degree of ultrastructural and enzymatic purity, with less than 1 percent intracellular residuum. They are produced in less than 1 second and remain as tightly adherent sheets on the surface on which the cells were grown. The cells are exposed to no agent other than nitrogen gas during the preparative procedure.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)