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Science 24 February 1978:
Vol. 199. no. 4331, pp. 906 - 907
DOI: 10.1126/science.199.4331.906
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Articles

Methylation of Humic Acid Fractions

R. L. WERSHAW 1 and D. J. PINCKNEY 1

1 U.S. Geological Survey, Denver Federal Center, Denver, Colorado 80225

A new three-step permethylation procedure for humic acid fractions has been developed. In the first step, carboxylic acid groups are methylated with diazomethane in dimethylformamide; in the second step, hydroxyl groups are methylated with methyl iodide and dimethyl sulfinyl carbanion in dimethylformamide; and in the third step, acidic groups hydrolyzed in the earlier steps are methylated with diazomethane. The resulting product is completely soluble in methylene chloride (a high degree of methylation has been achieved).

Submitted on September 27, 1977
Revised on November 15, 1977

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Science. ISSN 0036-8075 (print), 1095-9203 (online)