Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 16 December 1977:
Vol. 198. no. 4322, pp. 1175 - 1176
DOI: 10.1126/science.412254
Prev | Table of Contents | Next

Articles

Science, Vol 198, Issue 4322, 1175-1176
Copyright © 1977 by American Association for the Advancement of Science

articles

Characterization of bacterial growth by means of flow microfluorometry

JE Bailey, J Fazel-Makjlessi, DN McQuitty, YN Lee, JC Allred, and JA Oro

By means of flow microfluorometry, the protein and nucleic acid contents of individual bacterial cells may be measured at the rate of several thousand cells per second. Accumulation of such information over a few minutes yields the composition distribution of the microbial population. These distributions have been determined at different times during batch growth of Bactillus subtilis, and the results indicate that the variance of cell composition decreases as the population passes through the exponential into the stationary phase. The relative abundance of endospores and vegetative cells as well as the protein distributions of these subpopulations may be readily determined from flow microfluorometry data. Experimental access to such details of microbial population dynamics should foster improved understanding of cell growth, spore germination, and spore formation kinetics.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Limit of Resolution of Flow Cytometry for the Detection of Selected Bacterial Species.
M.S. Obernesser, S.S. Socransky, and P. Stashenko (1990)
Journal of Dental Research 69, 1592-1598
   Abstract »    PDF »
Bacterial characterization by flow cytometry.
M. Van Dilla, R. Langlois, D Pinkel, D Yajko, and W. Hadley (1983)
Science 220, 620-622
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)