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Science 12 August 1977:
Vol. 197. no. 4304, pp. 665 - 667
DOI: 10.1126/science.877580
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Articles

Science, Vol 197, Issue 4304, 665-667
Copyright © 1977 by American Association for the Advancement of Science

articles

Phenylketonuria: a new method for the simultaneous determination of plasma phenylalanine and tyrosine

RS Shen and CW Abell

This quantitative spectrophotometric method is based on the conversion of phenylalanine and tyrosine by phenylalanine ammonia-lyase to trans-cinnamic acid and trans-coumaric acid, respectively. Neither deproteinization nor prior incubation of the sample is required, and the entire procedure can be performed in 20 minutes. The method is sensitive to 1-micromolar concentrations of the two compounds, and only 20 microliters of plasma or serum is required to determine both phenylalanine and tyrosine simultaneously. These amino acids were determined between molar ratios (phenylalanine to tyrosine) of 0.1 to 40 in the serum or plasma of healthy individuals and plasma of phenylketonuric patients.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Extracorporeal Enzyme Reactors for Depletion of Phenylalanine in Phenylketonuria.
C. M. AMBRUS, S. ANTHONE, C. HORVATH, K. KALGHATGI, A. S. LELE, G. EAPEN, J. L. AMBRUS, A. J. RYAN, and P. LI (1987)
Ann Intern Med 106, 531-537
   Abstract »    PDF »
Phenylalanine depletion for the management of phenylketonuria: use of enzyme reactors with immobilized enzymes.
C. Ambrus, J. Ambrus, C Horvath, H Pedersen, S Sharma, C Kant, E Mirand, R Guthrie, and T Paul (1978)
Science 201, 837-839
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)