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Science 29 July 1977: Vol. 197. no. 4302, pp. 461 - 463 DOI: 10.1126/science.560061
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Articles
Science, Vol 197, Issue 4302, 461-463
Copyright © 1977 by American Association for the Advancement of Science
Primary bioassay of human tumor stem cells
AW Hamburger
and
SE Salmon
A simple method has been developed to support human tumor stem cell colony growth in soft agar. The technique appears suitable for culture of a variety of neoplasms of differing histopathology. Tumor stem cell colonies arising from different types of cancer have differing growth characteristics and colony morphology. This bioassay should be suitable for clinical studies of effects of anticancer drugs or irradiation on human tumor stem cells.
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