Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 15 July 1977:
Vol. 197. no. 4300, pp. 261 - 263
DOI: 10.1126/science.327543
Prev | Table of Contents | Next

Articles

Science, Vol 197, Issue 4300, 261-263
Copyright © 1977 by American Association for the Advancement of Science

articles

Mechanism of carbon isotope fractionation associated with lipid synthesis

MJ DeNiro and S Epstein

The low carbon-13/carbon-12 ratio of lipids is shown to result from isotopic fractionation during the oxidation of pyruvate to acetyl coenzyme A. In vitro analysis of the kinetic isotope effects of this reaction indicates that there will be a large, temperature-dependent difference in the carbon-13/carbon-12 ratio between the methyl and carbonyl carbon atoms of acetyl coenzyme A and between those carbon atoms of lipid components which derive from them.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stable carbon isotope discrimination by human 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
S. M. Ludke, U. Flenker, W. Schanzer, and D. Schomburg (2008)
J. Lipid Res. 49, 2620-2626
   Abstract »    Full Text »    PDF »
Stable carbon isotopes in exhaled breath as tracers for dietary information in birds and mammals.
C. C. Voigt, L. Baier, J. R. Speakman, and B. M. Siemers (2008)
J. Exp. Biol. 211, 2233-2238
   Abstract »    Full Text »    PDF »
Stable isotopes, ecological integration and environmental change: wolves record atmospheric carbon isotope trend better than tree rings.
J. K Bump, K. Fox-Dobbs, J. L Bada, P. L Koch, R. O Peterson, and J. A Vucetich (2007)
Proc R Soc B 274, 2471-2480
   Abstract »    Full Text »    PDF »
Oxidation rate and turnover of ingested sugar in hovering Anna's (Calypte anna) and rufous (Selasphorus rufus) hummingbirds.
K. C. Welch Jr and R. K. Suarez (2007)
J. Exp. Biol. 210, 2154-2162
   Abstract »    Full Text »    PDF »
Metabolic substrate use and the turnover of endogenous energy reserves in broad-tailed hummingbirds (Selasphorus platycercus).
S. A. Carleton, B. H. Bakken, and C. M. del Rio (2006)
J. Exp. Biol. 209, 2622-2627
   Abstract »    Full Text »    PDF »
Natural Abundance Carbon Isotope Composition of Isoprene Reflects Incomplete Coupling between Isoprene Synthesis and Photosynthetic Carbon Flow.
H. P. Affek and D. Yakir (2003)
Plant Physiology 131, 1727-1736
   Abstract »    Full Text »    PDF »
Lipid-Like Material as the Source of the Uncharacterized Organic Carbon in the Ocean?.
J. Hwang and E. R. M. Druffel (2003)
Science 299, 881-884
   Abstract »    Full Text »    PDF »
Metabolic Origin of Carbon Isotope Composition of Leaf Dark-Respired CO2 in French Bean.
G. Tcherkez, S. Nogues, J. Bleton, G. Cornic, F. Badeck, and J. Ghashghaie (2003)
Plant Physiology 131, 237-244
   Abstract »    Full Text »    PDF »
The Analysis of 13C/12C Ratios in Exhaled CO2: Its Advantages and Potential Application to Field Research to Infer Diet, Changes in Diet Over Time, and Substrate Metabolism in Birds.
K. A. Hatch, B. Pinshow, and J. R. Speakman (2002)
Integr. Comp. Biol. 42, 21-33
   Abstract »    Full Text »    PDF »
Fractionation of Carbon and Hydrogen Isotopes in Biosynthetic Processes.
J. M. Hayes and J. M. Hayes (2001)
Reviews in Mineralogy and Geochemistry 43, 225-277
   Full Text »    PDF »
Isotopic Biogeochemistry of Marine Organic Carbon.
K. H. Freeman and K. H. Freeman (2001)
Reviews in Mineralogy and Geochemistry 43, 579-605
   Full Text »    PDF »
Evidence of Substantial Carbon Isotope Fractionation among Substrate, Inorganic Carbon, and Biomass during Aerobic Mineralization of 1,2-Dichloroethane by Xanthobacter autotrophicus.
D. Hunkeler and R. Aravena (2000)
Appl. Envir. Microbiol. 66, 4870-4876
   Abstract »    Full Text »
Ratios of Carbon Isotopes in Microbial Lipids as an Indicator of Substrate Usage.
W.-R. Abraham, C. Hesse, and O. Pelz (1998)
Appl. Envir. Microbiol. 64, 4202-4209
   Abstract »    Full Text »
Stable-Carbon-Isotope Composition of Fatty Acids in Hydrothermal Vent Mussels Containing Methanotrophic and Thiotrophic Bacterial Endosymbionts.
D. W. Pond, M. V. Bell, D. R. Dixon, A. E. Fallick, M. Segonzac, and J. R. Sargent (1998)
Appl. Envir. Microbiol. 64, 370-375
   Abstract »    Full Text »
Carbon Isotope Effects on the Fructose-1,6-bisphosphate Aldolase Reaction, Origin for Non-statistical 13C Distributions in Carbohydrates.
G. Gleixner and H.-L. Schmidt (1997)
J. Biol. Chem. 272, 5382-5387
   Abstract »    Full Text »    PDF »
A Molecular Organic Carbon Isotope Record of Miocene Climate Changes.
M. Schoell, M. Schoell, S. Schouten, J. S. S. Damste, J. W. de Leeuw, and R. E. Summons (1994)
Science 263, 1122-1125
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)