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Science 17 June 1977:
Vol. 196. no. 4296, pp. 1313 - 1319
DOI: 10.1126/science.325648
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Articles

Science, Vol 196, Issue 4296, 1313-1319
Copyright © 1977 by American Association for the Advancement of Science

articles

Rat insulin genes: construction of plasmids containing the coding sequences

A Ullrich, J Shine, J Chirgwin, R Pictet, E Tischer, WJ Rutter, and HM Goodman

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.


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