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Science 27 May 1977:
Vol. 196. no. 4293, pp. 992 - 994
DOI: 10.1126/science.558652
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Articles

Science, Vol 196, Issue 4293, 992-994
Copyright © 1977 by American Association for the Advancement of Science

articles

African trypanosomes: cultivation of animal-infective Trypanosoma brucei in vitro

H Hirumi, JJ Doyle, and K Hirumi

Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37 degrees C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.


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