Science, Vol 196, Issue 4286, 202-205
Copyright © 1977 by American Association for the Advancement of Science
Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis
MI Lomax,
RB Helling,
LI Hecker,
SD Schwartzbach,
and
WE Barnett
Fragments of Euglena chloroplast DNA generated by endonuclease R-Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA, Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.