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Science 8 April 1977:
Vol. 196. no. 4286, pp. 200 - 202
DOI: 10.1126/science.847468
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Articles

Science, Vol 196, Issue 4286, 200-202
Copyright © 1977 by American Association for the Advancement of Science

articles

Human globin messenger RNA: importance of cloning for structural analysis

JT Wilson, BG Forget, LB Wilson, and SM Weissman

The sequence of most of the human beta globin messenger RNA and large sections of the alpha globin messenger RNA has been determined. Partly because of genetic polymorphism, it was necessary to clone globin complementary DNA in order to extend the analysis. Purified human fetal globin messenger RNA was isolated and used as a template by reverse transcriptase to produce duplex complementary DNA molecules. These molecules were linked in vitro to plasmid DNA by use of T4 ligase in the presence of Escherichia coli Pol 1. Several colonies transformed by these molecules have been shown to hybridize with labeled human globin complementary RNA.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)