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Science 8 April 1977:
Vol. 196. no. 4286, pp. 177 - 180
DOI: 10.1126/science.847463
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Articles

Science, Vol 196, Issue 4286, 177-180
Copyright © 1977 by American Association for the Advancement of Science

articles

Chemical synthesis of restriction enzyme recognition sites useful for cloning

RH Scheller, RE Dickerson, HW Boyer, AD Riggs, and K Itakura

By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.


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A technique for expressing eukaryotic genes in bacteria.
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Insertion Mutations Affecting Transposition of Tn3 and Replication of a ColE1 Derivative.
F. Heffron, M. So, and B. J. McCarthy (1979)
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Rat insulin genes: construction of plasmids containing the coding sequences.
A Ullrich, J Shine, J Chirgwin, R Pictet, E Tischer, W. Rutter, and H. Goodman (1977)
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Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites.
R. Scheller, T. Thomas, A. Lee, W. Klein, W. Niles, R. Britten, and E. Davidson (1977)
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