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Science 8 April 1977:
Vol. 196. no. 4286, pp. 175 - 177
DOI: 10.1126/science.322278
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Articles

Science, Vol 196, Issue 4286, 175-177
Copyright © 1977 by American Association for the Advancement of Science

articles

EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system

P Leder, D Tiemeier, and L Enquist

A derivative of bacteriophage lambda has been modified and tested together with an appropriate host system to meet the criteria of EK2 biologic containment for cloning DNA from higher organisms. In this report certain of the safety features are summarized and some of the tests carried out to confirm the containment properties of the vector are described. The cloning efficiency of this system, together with available gene purification and hybrid screening technology, indicate that it can be used to clone DNA fragments carrying specific, unique mammalian genes.


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