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Science 11 March 1977:
Vol. 195. no. 4282, pp. 996 - 998
DOI: 10.1126/science.841325
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Articles

Science, Vol 195, Issue 4282, 996-998
Copyright © 1977 by American Association for the Advancement of Science

articles

Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component

DG Allen, Blinks JR, and FG Prendergast

Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10(-9) to 10(-2) molar. At very low Ca2+ concentations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.


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