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Science 4 February 1977:
Vol. 195. no. 4277, pp. 495 - 497
DOI: 10.1126/science.835010
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Articles

Science, Vol 195, Issue 4277, 495-497
Copyright © 1977 by American Association for the Advancement of Science

articles

Calcium-induced displacement of membrane-associated particles upon aggregation of chromaffin granules

R Schober, C Nitsch, U Rinne, and SJ Morris

Isolated chromaffin granules incubated in 10 millimolar calcium chloride aggregated, forming contact sites with a pentalaminar membrane structure. These circular attachment sites were free of membrane-associated particles, which accumulated at the periphery. Incubation in 20 millimolar ethylenediaminetetraacetic acid reversed these changes, which are regarded as initial events in the membrane fusion reaction.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)