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Science 28 January 1977:
Vol. 195. no. 4276, pp. 393 - 394
DOI: 10.1126/science.318764
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Articles

Science, Vol 195, Issue 4276, 393-394
Copyright © 1977 by American Association for the Advancement of Science

articles

Plasmid detection and sizing in single colony lysates

WM Barnes

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Preparation of Plasmid DNA: Toothpick Minipreparation.
J. Sambrook and D. W. Russell (2006)
CSH Protocols 2006, pdb.prot3905
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Characterization and molecular cloning of a human parvovirus genome.
S. Cotmore and P Tattersall (1984)
Science 226, 1161-1165
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)