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Science 26 November 1976:
Vol. 194. no. 4268, pp. 951 - 953
DOI: 10.1126/science.62397
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Articles

Science, Vol 194, Issue 4268, 951-953
Copyright © 1976 by American Association for the Advancement of Science

articles

Revertants of human cells transformed by murine sarcoma virus

HY Cho, EC Cutchins, JS Rhim, and RJ Huebner

Revertants of nonproducer human osteosarcoma (NP/KHOS) cells induced by Kirsten murine sarcoma virus were isolated after incubating at high temperature (40.5 degrees C) overnight and subcloning at 36 degrees C. The morphologic variants, from which murine sarcoma virus could no longer be rescued, had growth properties similar to those of the nontransformed, parent human osteosarcoma cells and did not release RNA-dependent DNA polymerase activity. These revertants were nontumorigenic in nude mice. The revertants supported leukemia virus growth and showed an enhanced sensitivity to murine sarcoma virus superinfection. Thus, the revertants were from human cells transformed by an oncogenic RNA virus.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cycloheximide-dependent reversion of human cells transformed by MSV and chemical carcinogen.
H. Cho and J. Rhim (1979)
Science 205, 691-693
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