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Science 19 November 1976:
Vol. 194. no. 4267, pp. 855 - 857
DOI: 10.1126/science.982049
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Articles

Science, Vol 194, Issue 4267, 855-857
Copyright © 1976 by American Association for the Advancement of Science

articles

Radioimmunoassay for creatine kinase isoenzymes

R Roberts, BE Sobel, and CW Parker

Creatine kinase has three isoenzymes designated MM, MB, and BB, with BB being the grain form and MM the muscle form. Antibodies to BB creatine kinase were obtained by immunization of rabbits with human BB creatine kinase. The antibodies demonstrated specificity for BB and MB creatine kinase (myocardial isoenzyme) but no cross-reactivity with MM creatine kinase. With the use of this antibody, a highly sensitive radioimmunoassay capable of measuring picomolar amounts of MB creatine kinase has been developed. Clinical application of this method should provide a sensitive and specific test for the diagnosis of myocardial infarction.


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Cardiac Biomarkers for Detection of Myocardial Infarction: Perspectives from Past to Present.
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Subforms of Creatine Kinase MB in the Diagnosis of Myocardial Infarction.
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Reduction of Myocardial Infarct Size: Approach for the 1980s.
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