Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 24 September 1976:
Vol. 193. no. 4259, pp. 1254 - 1255
DOI: 10.1126/science.183265
Prev | Table of Contents | Next

Articles

Science, Vol 193, Issue 4259, 1254-1255
Copyright © 1976 by American Association for the Advancement of Science

articles

Localization of apolipoprotein B in intestinal epithelial cells

RM Glickman, J Khorana, and A Kilgore

Indirect immunofluorescence techniques were employed to determine the distribution within intestinal epithelial cells of apolipoprotein B, a protein essential for the normal transport of fat. Isolated intestinal cells were prepared from rats either during active lipid absorption or after biliary diversion. Specific immunofluorescence from an antiserum to apolipoprotein B was detected in the apical portion of epithelial cells from bile-diverted animals, demonstrating that a pool of apolipoprotein B is present in the nonabsorptive epithelial cell and may be a component of intestinal cell membranes. During lipid absorption in normal rats, an early and sustained increase in immunofluorescence was demonstrated, consistent with an increase synthesis of apolipoprotein B during lipid absorption. This study demonstrates the presence of apolipoprotein B within intestinal epithelium and provides evidence for the participation of this apoprotein in intestinal lipid transport.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Intestinal Lipoprotein Production Is Stimulated by an Acute Elevation of Plasma Free Fatty Acids in the Fasting State: Studies in Insulin-Resistant and Insulin-Sensitized Syrian Golden Hamsters.
G. F. Lewis, M. Naples, K. Uffelman, N. Leung, L. Szeto, and K. Adeli (2004)
Endocrinology 145, 5006-5012
   Abstract »    Full Text »    PDF »
Lipid-dependent Bidirectional Traffic of Apolipoprotein B in Polarized Enterocytes.
E. Morel, S. Demignot, D. Chateau, J. Chambaz, M. Rousset, and F. Delers (2004)
Mol. Biol. Cell 15, 132-141
   Abstract »    Full Text »    PDF »
Expression of intrinsic factor and pepsinogen in the rat stomach identifies a subset of parietal cells.
J.-S. Shao, W. Schepp, and D. H. Alpers (1998)
Am J Physiol Gastrointest Liver Physiol 274, G62-G70
   Abstract »    Full Text »    PDF »
Serum lipoprotein concentrations in cystic fibrosis.
W. Vaughan, F. Lindgren, J. Whalen, and S Abraham (1978)
Science 199, 783-786
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)