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Science 10 September 1976:
Vol. 193. no. 4257, pp. 1021 - 1023
DOI: 10.1126/science.1085035
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Articles

Science, Vol 193, Issue 4257, 1021-1023
Copyright © 1976 by American Association for the Advancement of Science

articles

1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay

JA Eisman, AJ Hamstra, BE Kream, and HF DeLuca

A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients.


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Bone Disease in Alcohol Abuse.
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The Development of Pseudohypoparathyroidism: Involvement of Progressively Increasing Serum Parathyroid Hormone Concentrations, Increased 1,25-Dihydroxyvitamin D Concentrations, and 'Migratory' Subcutaneous Calcifications.
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Arch Pediatr Adolesc Med 138, 654-658
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Secondary Hyperparathyroidism and Bone Disease in Infants Receiving Long-term Furosemide Therapy.
P. S. Venkataraman, B. K. Han, R. C. Tsang, and C. C. Daugherty (1983)
Arch Pediatr Adolesc Med 137, 1157-1161
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Calcifediol (25-Hydroxyvitamin D3) in the Treatment of Uremic Bone Disease.
S. L. TEITELBAUM (1981)
Ann Intern Med 94, 404-405
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Serum 1,25-Dihydroxyvitamin D Levels in Normal Children and in Vitamin D Disorders.
R. W. Chesney, J. F. Rosen, A. J. Hamstra, and H. F. DeLuca (1980)
Arch Pediatr Adolesc Med 134, 135-139
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Evidence for extra-renal 1 alpha-hydroxylation of 25-hydroxyvitamin D3 in pregnancy.
T. Gray, G. Lester, and R. Lorenc (1979)
Science 204, 1311-1313
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Adequate response of plasma 1,25-dihydroxyvitamin D to parturition in paretic (milk fever) dairy cows.
R. Horst, J. Eisman, N. Jorgensen, and H. DeLuca (1977)
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