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Science 10 September 1976:
Vol. 193. no. 4257, pp. 1021 - 1023
DOI: 10.1126/science.1085035
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Articles

Science, Vol 193, Issue 4257, 1021-1023
Copyright © 1976 by American Association for the Advancement of Science

articles

1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay

JA Eisman, AJ Hamstra, BE Kream, and HF DeLuca

A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cloning of Human 25-Hydroxyvitamin D-1{alpha}-Hydroxylase and Mutations Causing Vitamin D-Dependent Rickets Type 1.
G. K. Fu, D. Lin, M. Y. H. Zhang, D. D. Bikle, C. H. L. Shackleton, W. L. Miller, and A. A. Portale (1997)
Mol. Endocrinol. 11, 1961-1970
   Abstract »    Full Text »    PDF »
Evidence for extra-renal 1 alpha-hydroxylation of 25-hydroxyvitamin D3 in pregnancy.
T. Gray, G. Lester, and R. Lorenc (1979)
Science 204, 1311-1313
   Abstract »    PDF »
Adequate response of plasma 1,25-dihydroxyvitamin D to parturition in paretic (milk fever) dairy cows.
R. Horst, J. Eisman, N. Jorgensen, and H. DeLuca (1977)
Science 196, 662-663
   Abstract »    PDF »


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