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Science 10 September 1976: Vol. 193. no. 4257, pp. 1021 - 1023 DOI: 10.1126/science.1085035
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Articles
Science, Vol 193, Issue 4257, 1021-1023
Copyright © 1976 by American Association for the Advancement of Science
1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay
JA Eisman,
AJ Hamstra,
BE Kream,
and
HF DeLuca
A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients.
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