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Science 13 August 1976:
Vol. 193. no. 4253, pp. 591 - 592
DOI: 10.1126/science.959819
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Articles

Science, Vol 193, Issue 4253, 591-592
Copyright © 1976 by American Association for the Advancement of Science

articles

The insertion of DNA into vaccinia virus

C Morgan

Cells infected with vaccinia virus in the presence of hydroxyurea (HU), which blocks DNA replication, were examined in thin sections by electron microscopy at intervals after removal of HU. Dense, fibrillar material was observed at the orifice formed just before closure of the membrane constituting the envelope of the immature form of the virus. It is concluded that synchrony of assembly enabled stages in the condensation and insertion of viral deoxyribonucleoprotein to be observed. The mechanism appears to be similar to that encountered in morphologic studies of herpes simplex virus and in biochemical studies of poliovirus, adenovirus, and several bacteriophages.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Major Core Protein P4a (A10L Gene) of Vaccinia Virus Is Essential for Correct Assembly of Viral DNA into the Nucleoprotein Complex To Form Immature Viral Particles.
R. Heljasvaara, D. Rodriguez, C. Risco, J. L. Carrascosa, M. Esteban, and J. R. Rodriguez (2001)
J. Virol. 75, 5778-5795
   Abstract »    Full Text »    PDF »
DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles.
M. C. Cassetti, M. Merchlinsky, E. J. Wolffe, A. S. Weisberg, and B. Moss (1998)
J. Virol. 72, 5769-5780
   Abstract »    Full Text »    PDF »
Repression of the A8L Gene, Encoding the Early Transcription Factor 82-Kilodalton Subunit, Inhibits Morphogenesis of Vaccinia Virions.
X. Hu, E. J. Wolffe, A. S. Weisberg, L. J. Carroll, and B. Moss (1998)
J. Virol. 72, 104-112
   Abstract »    Full Text »    PDF »
The Role of a 21-kDa Viral Membrane Protein in the Assembly of Vaccinia Virus from the Intermediate Compartment.
J. Krijnse-Locker, S. Schleich, D. Rodriguez, B. Goud, E. J. Snijder, and G. Griffiths (1996)
J. Biol. Chem. 271, 14950-14958
   Abstract »    Full Text »    PDF »


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