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Science 13 August 1976:
Vol. 193. no. 4253, pp. 587 - 588
DOI: 10.1126/science.959817
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Articles

Science, Vol 193, Issue 4253, 587-588
Copyright © 1976 by American Association for the Advancement of Science

articles

Intracellular 5-Phosphoribosyl-1-Pyrophosphate: Decreased Availability During Glutamine Limitation

SD Skaper, RC Willis, and JE Seegmiller

Culture of human lymphoblasts in medium containing limited glutamine (less than 0.2 millimolar) effects an inhibition of purine base reutilization and synthesis de novo. Both processes appear to be inhibited by a decreased availability of the common substrate 5-phosphoribosyl-J-pyrophosphate. Under conditions of glutamine limitation, the maintenance of capacity for purine base utilization and synthesis de novo and consequently growth was dependent on supplementation of cultures with inosine. The purine nucleoside provides a source of ribose phosphate for synthesis of 5-phosphoribosyl-J-pyrophosphate. Glutamine limitation may result in a decreased availability of ribose-5-phosphate.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stimulation of Protein, RNA, and Nucleotide Synthesis in Lymphocytes After Abdominal Surgery Is Not Affected by Postoperative Amino Acid Supply.
T. Chikenji, M. Mizutani, H. Furukawa, and Y. Kitsukawa (1991)
JPEN J Parenter Enteral Nutr 15, 371-375
   Abstract »    PDF »
Phosphoribosylpyrophosphate synthesis in cultured human cells.
P. Benke and D Dittmar (1977)
Science 198, 1171-1173
   Abstract »    PDF »


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