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Science 28 May 1976:
Vol. 192. no. 4242, pp. 898 - 900
DOI: 10.1126/science.179138
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Articles

Science, Vol 192, Issue 4242, 898-900
Copyright © 1976 by American Association for the Advancement of Science

articles

Characterization of the androgen receptor from a Syrian hamster ductus deferens tumor cell line (DDT1)

JS Norris and PO Kohler

The hamster ductus deferens cloned tumor cell line (DDT1) contains a complex steroid receptor protein that binds 3H-labeled 5 alpha-dihydrotestosterone,. Diethylaminoethyl (DEAE) chromatography of cytosol from these cells yields two major receptor peaks of activity. Identification of this steroid binding protein as a cytoplasmic receptor was confirmed by salt dissociation on sucrose gradients, stability of the hormone-receptor complex at 0 degrees C, and the retention patterns on phosphocellulose and DEAE cellulose. Multiple forms of the receptor exist in a single homogeneous cell type. The data support the theory that steroid hormones bind to a cytoplasmic protein receptor composed of dissimilar subunits as the initial step in steroid hormone action.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Exogenous 17ß-Estradiol Blocks Alpha and Mu but Not Pi Class Glutathione S-Transferase Immunoreactivity in Epithelium of Syrian Hamster Vas Deferens.
C. E. Hudson, J. E. DeHaven, B. A. Schulte, and J. S. Norris (1999)
J. Histochem. Cytochem. 47, 91-98
   Abstract »    Full Text »
Molecular Cloning of TA16, a Transcriptional Repressor That May Mediate Glucocorticoid-Induced Growth Arrest of Leiomyosarcoma Cells.
W. Fan, J.-X. Ma, L. Cheng, and J. S. Norris (1997)
Mol. Endocrinol. 11, 1342-1352
   Abstract »    Full Text »


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