Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 January 1976:
Vol. 191. no. 4222, pp. 96 - 98
DOI: 10.1126/science.1246601
Prev | Table of Contents | Next

Articles

Science, Vol 191, Issue 4222, 96-98
Copyright © 1976 by American Association for the Advancement of Science

articles

HeLa cultures defined

WA Nelson-Rees and RR Flandermeyer

A list is presented of references to all known publications on properties which have served to relate strains of HeLa cells to each other as well as to indict other purported human cell lines as HeLa cell contaminants. Eleven additional cell lines not previously indicted are described. When they exhibit (i) type A (fast) mobility for glucose-6-phosphate dehydrogenase, (ii) phosphoglucomutase type 1 at locus 1 and locus 3, (iii) absence of a Y chromosome by fluorescent staining, and (iv) possession of a complex of trypsin-Giemsa banded marker chromosomes present in known HeLa cells, then cell substrates regardless of designation should be considered de facto strains of HeLa.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
High-throughput detection and multiplex identification of cell contaminations.
M. Schmitt and M. Pawlita (2009)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
A bioinformatics analysis of the cell line nomenclature.
S. Sarntivijai, A. S. Ade, B. D. Athey, and D. J. States (2008)
Bioinformatics 24, 2760-2766
   Abstract »    Full Text »    PDF »
Cell culture forensics.
S. J. O'Brien (2001)
PNAS 98, 7656-7658
   Full Text »    PDF »
Identification of a Novel Tumor-Specific CTL Epitope Presented by RMA, EL-4, and MBL-2 Lymphomas Reveals Their Common Origin.
T. van Hall, J. van Bergen, P. A. van Veelen, M. Kraakman, L. C. Heukamp, F. Koning, C. J. M. Melief, F. Ossendorp, and R. Offringa (2000)
J. Immunol. 165, 869-877
   Abstract »    Full Text »    PDF »
Measles- or Mumps Virus-Infected Cells Forming Rosettes With Lymphocytes From Patients With Multiple Sclerosis.
L. H. Tobler, K. P. Johnson, and G. C. Buehring (1982)
Arch Neurol 39, 565-569
   Abstract »    PDF »
Cross-contamination of cells in culture.
W. Nelson-Rees, D. Daniels, and R. Flandermeyer (1981)
Science 212, 446-452
   Abstract »    PDF »
T-1 cells are HeLa and not of normal human kidney origin.
W. Nelson-Rees, R. Flandermeyer, and D. Daniels (1980)
Science 209, 719-720
   PDF »
Inter- and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures.
W. Nelson-Rees and R. Flandermeyer (1977)
Science 195, 1343-1344
   Abstract »    PDF »
Enzyme polymorphisms as genetic signatures in human cell cultures.
S. O'Brien, G Kleiner, R Olson, and J. Shannon (1977)
Science 195, 1345-1348
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)