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Science 19 December 1975:
Vol. 190. no. 4220, pp. 1226 - 1228
DOI: 10.1126/science.1060178
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Articles

Science, Vol 190, Issue 4220, 1226-1228
Copyright © 1975 by American Association for the Advancement of Science

articles

Molecular vehicle properties of the broad host range plasmid RK2

R Meyer, D Figurski, and DR Helinski

The pladmid RK2 is stably maintained in a broad range of gram-negative bacteria. The RK2 DNA has a single Eco RI restriction site. The insertion of a DNA fragment into this site does not interfere with either plasmid maintenance or self-transmissibility. Because RK2 has a broad host range, it should be useful for the construction in vitro of hybrid plasmid molecules capable of being established by conjugal transfer or transformation into many genera of gram-negative organisms.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Bacterial partitioning proteins affect the subcellular location of broad-host-range plasmid RK2.
K. Kolatka, M. Witosinska, M. Pierechod, and I. Konieczny (2008)
Microbiology 154, 2847-2856
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Science. ISSN 0036-8075 (print), 1095-9203 (online)