Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 19 December 1975:
Vol. 190. no. 4220, pp. 1204 - 1206
DOI: 10.1126/science.1198106
Prev | Table of Contents | Next

Articles

Science, Vol 190, Issue 4220, 1204-1206
Copyright © 1975 by American Association for the Advancement of Science

articles

Calcium ion distribution in cytoplasm visualised by aequorin: diffusion in cytosol restricted by energized sequestering

B Rose and WR Loewenstein

The distribution of Ca2+ in the cytoplasm following a local rise in Ca2+ concentration is visualized by means of aequorin luminescence and a television system with an image intensifier. Diffusion of Ca2+ through the cytosol is so constrained that a rise in cytoplasmic Ca2+ concentration produced by local Ca2+ entry through cell membrane or by local Ca2+ injection is confined to the immediate vicinity of these sites. The diffusion constraints are lifted by treatment with cyanide or ruthenium red. Thus, energized calcium sequestering, probably by mitochondria, is the dominant factor in the constraints. In cell regions where the sequestering machinery is sufficiently dense, different Ca2+ message functions inside a cell may be effectively segregated, permitting private-line intracellular communication.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mitochondrial Transport of Cations: Channels, Exchangers, and Permeability Transition.
P. Bernardi (1999)
Physiol Rev 79, 1127-1155
   Abstract »    Full Text »    PDF »
Roles of Ca2+, inositol trisphosphate and cyclic ADP-ribose in mediating intercellular Ca2+ signaling in sheep lens cells.
G. Churchill and C. Louis (1998)
J. Cell Sci. 111, 1217-1225
   Abstract »    PDF »
Spatially nonuniform changes in intracellular calcium ion concentrations.
H. Harary and J. Brown (1984)
Science 224, 292-294
   Abstract »    PDF »
Secretion in mast cells induced by calcium entrapped within phospholipid vesicles.
T. Theoharides and W. Douglas (1978)
Science 201, 1143-1145
   Abstract »    PDF »
Permeable Junctions.
W. R. Loewenstein (1976)
Cold Spring Harb Symp Quant Biol 40, 49-63
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)